Evaluation of bioavailability of antioxidants in EpiCor at the cellular level and in clinical studies, using the cell-based antioxidant protection of erythrocytes (CAP-e) bioassay

Purpose of the study

The objective of this study was to evaluate EpiCor» antioxidant bioavailability.  One aspect of bioavailability is to examine whether compounds are capable of crossing the cell membrane and entering live cells. Performing cell-based testing for antioxidant protection involves the dynamics Of living cells, their enzymes, and membrane properties.

An improvement in serum antioxidant protection capacity after ingestion of a food or natural product may reflect the content of easily absorbed antioxidants existing in the native product, as well as antioxidant compounds released or generated as a result of normal digestive processes. Unknown metabolites may contribute significantly to the in vivo antioxidant protection. By using the CAP-e assay to evaluate the serum capacity for antioxidant protection, such metabolites do not need to be chemically identified; as long as they are able to enter and protect living cells from oxidative damage they contribute to the CAP-e results. This is in contrast to other types of bioavailability studies, where chemical analysis is performed on serum to examine the sample for metabolites known to have come from the digestion of a natural product.

Poster (pdf)