Project Type: Scientific Posters


Rapid increase in fibrinolytic capacity of human blood after consumption of a blend of oral enzymes and anti-inflammatory fruit- and berry-extracts.

Objective

To present a study design evaluation of immediate changes in blood clot-lysing capacity after consumption of a blend of anti-inflammatory fruit-and berry-extracts and oral enzymes.

Poster (pdf)


Cell-based Antioxidant Protection. A novel method for evaluation of natural products

Abstract

The objective of this research was to examine the value of a novel cell-based antioxidant protection in erythrocytes (CAP-e)* assay for the evaluation of whether antioxidants in natural products are capable of penetrating and protecting live cells from oxidative damage.

The assay uses basic chemical principles, similar to those used with the ORAC test, to measure specific effects of products on a highly simplistic cell type, the erythrocyte (red blood cell). The advantage of utilizing the erythrocyte for the assay is that this cell type does not perform cellular signaling, undergo apoptosis, or produce reactive oxygen species, whereas all other cell types used in cell-based assays can.

We have tested natural products with known ORAC values, and found that certain products such as the freeze-dried Acai berry, with an extremely high ORAC of 1,027 μmol Trolox equivalents per gram, also provided good protection of cells from oxidative damage. We have also identified a bovine colostrum whey-based extract, Immunel, with a known ORAC value of 18 μmol Trolox equivalents per gram, which was able to protect cells better than would be expected based on the product’s ORAC value alone.

Furthermore, we used a version of the CAP-e method to test serum samples from a clinical pilot study, testing antioxidant uptake after consumption of placebo versus MonaVie Active, a juice rich in Acai. The CAP-e assay was able to detect an increase in serum antioxidants, and the data correlated with changes in lipid peroxidation as measured by the T BARS test.

We are currently performing a single laboratory validation for the assay, using a select panel of natural products of plant, animal, and microbial origins.

Poster (pdf)


Pain reduction and improvement of range of motion after consumption of MonaVie Active, an Acai-rich fruit/berry juice blend.

Abstract

The objective was to evaluate the impact of consumption of an Acai-rich fruit/berry juice blend on pain and range of motion. An open-label clinical pilot study was performed by recruiting 14 study participants with some limitations in range of motion associated with pain and affecting their activities of daily living.

The study participants were supplied with juice for 12 weeks, and instructed to consume four ounces (120 mL) daily for the duration of the study. The study participants went through a medical exam at study entry and exit, and were assessed by a nurse (structured interview, questionnaires, blood samples), and a chiropractor (range of motion assessment). The pain levels were scored using a visual analogue scale (VAS).  The ROM assessment was performed using dual digital inclinometry, using the J-Tech wireless system as recommended by the American Medical Association guidelines.

Serum testing showed a significant increase in antioxidant activity already after 2 weeks of consumption, using the CAP-e cell-based antioxidant protection assay. A reduction of inflammation was seen as a reduction of C-Reactive Protein (CRP).

Poster (pdf)