Project Type: Scientific Posters


Antioxidant and Immune-Modulatory Effects of Aloe vera Decolorized Leaf Juice vs. Inner Leaf Juice

The aloe vera (Aloe vera) plant has a long history of traditional therapeutic topical use. Its leaf and inner leaf juices are also ingested in various forms. Aloe vera leaf juice is made from macerated aloe leaves, and when it is intended for human ingestion, it is purified of latex constituents via a charcoal filtration process known as decolorization. Several publications have shown immunomodulatory effects, as well as antibacterial, antifungal and anti-parasitic properties of aloe vera inner leaf juice, but limited information is available regarding these effects for aloe vera leaf juice after the decolorization process. The aim of this preliminary study was to compare biologically-relevant antioxidant and immune-modulatory effects of aloe vera inner leaf and decolorized leaf preparations using a variety of in vitro techniques.

ExpBio poster 5-1-17

FASEB JOURNAL


Topical effects of a novel blend of natural products (DermaStem) on human skin.

Objective

The objective for this research was to document the effects of DermaStem on specific modes Of action on primary human dermal  fibroblasts and inflammatory cells in vitro, and on hydration status, skin elasticity, and wrinkle reduction in healthy human facial skin in vivo.

Poster (pdf)


Analgesic and anti-inflammatory properties of a phycocyanin-enriched microalgae extract CyaninPlus.

Objective and methodology

The objective for this research was to document the anti-inflammatory  properties Of the microalgae extract in vitro and in vivo.

A panel of in vitro tests addressed the following:
  • Antioxidant capacity
  • Inhibition of inflammatory enzymes COX-2 and Lipoxygenase
  • Reduction of free radical production by inflammatory cells
Two human clinical pilot studies were performed:
  • An open-label 4-week study on 12 people 40-70 years of age suffering from chronic joint pain
  • A double-blinded placebo-controlled cross-over study involving 12 people in the same age range, where each consumption phase was 1 week, separated by a wash-out period of one week.

Poster (pdf)


Effects of Ovacore, a water-soluble egg membrane hydrolysate, on reduced inflammation, relief of joint pain, and support of skin health.

Aim

The goal for this study was to evaluate anti-inflammatory and structural tissue support of joints, microvessels, and skin by the water-soluble, natural egg membrane hydrolyzate Ovacore.

Poster (pdf)


Significant improvements in blood lipid profile after 2 weeks consumption of Wholemega, a salmon oil of wild-caught Alaskan salmon, containing a broad-spectrum of omega fatty acids.

Purpose

The purpose of this study was to evaluate the effects of Wholemega consumption on blood lipids, using a double-
blinded, randomized, placebo-controlled study design, to explore the effects of consumption on HDL cholesterol levels, LDL levels
and particle size, and triglycerides.

Due to the multiple negative health impacts of elevated levels of ultra-small LDL particles and triglycerides, it was of particular
interest to examine whether consumption of Wholemega would support beneficial changes over a short study period.

Poster (pdf)


Support of anti-bacterial and anti-viral innate immune functions in vitro and in vivo by Immunel, a novel extract from bovine colostrum whey.

Background

Colostrum is the biological fluid produced from the mammary glands of female mammals shortly after giving birth. The fluid has a distinct composition different from the milk produced afterwards. The composition Of colostrum provides immediate immune protection to the newborn. The supportive properties of bovine colostrum when consumed by other mammalian species, including pigs and humans, are well documented in the literature. The immune modulating compounds in colostrum from farm animals have been receiving attention
in a number of clinical applications. Immunel is an immunoglobulin-depleted lactose-reduced extract from colostral whey (bovine).

Poster (pdf)


Evaluation of bioavailability of antioxidants in EpiCor at the cellular level and in clinical studies, using the cell-based antioxidant protection of erythrocytes (CAP-e) bioassay

Purpose of the study

The objective of this study was to evaluate EpiCor» antioxidant bioavailability.  One aspect of bioavailability is to examine whether compounds are capable of crossing the cell membrane and entering live cells. Performing cell-based testing for antioxidant protection involves the dynamics Of living cells, their enzymes, and membrane properties.

An improvement in serum antioxidant protection capacity after ingestion of a food or natural product may reflect the content of easily absorbed antioxidants existing in the native product, as well as antioxidant compounds released or generated as a result of normal digestive processes. Unknown metabolites may contribute significantly to the in vivo antioxidant protection. By using the CAP-e assay to evaluate the serum capacity for antioxidant protection, such metabolites do not need to be chemically identified; as long as they are able to enter and protect living cells from oxidative damage they contribute to the CAP-e results. This is in contrast to other types of bioavailability studies, where chemical analysis is performed on serum to examine the sample for metabolites known to have come from the digestion of a natural product.

Poster (pdf)


Evaluation of a novel probiotic Bacillus coagulans (Ganeden BC30) cell wall and metabolites: Anti-infammatory and immune modulating effects in vitro.

Purpose of this study

This study was undertaken to evaluate whether the biological effects caused by the novel probiotic, spore-forming strain of Bacillus coagulans GBI-30 (PTA-6086, Ganeden BC30) were due to cell wall components alone, or whether secreted substances from the live bacteria had additional biological properties. This investigation was conducted to address specific aspects of the ongoing discussion as to whether probiotics only act via the cell walls, or whether they may provide different bioactive compounds when alive and metabolically active.

Poster (pdf)


Analgesic and anti-inflammatory properties of a novel phycocyanin-rich microalgae extract, CyaninPlus.

CyaninPlus is an extract from microalgae. Its main component is Phycocyanin (PC), a known selective COX-2 inhibitor. with cardio- and neuro-protective effects. In addition to PC, CyaninPlus contains other anti-inflammatory compounds, different from PC, and with complementary anti-inflammatory properties.

The objective of this pilot study was to evaluate the anti-inflammatory properties of CyaninPlus in vitro, and conduct a clinical pilot study on the pain reducing effects when
human subjects consumed 1 gram daily.

Poster (pdf)


The CAP-e assay: Testing antioxidant bioavailability at the cellular level and creating a foundation for further biological testing of natural products.

Objective

To present recent advances using the CAP-e cell-based antioxidant protection assay, with regards to its role in natural products testing, its application as a QC tool, and the importance of controlling for inter-assay variability between batches Of cells used for the assay.

Summary

We have developed a novel accelerated method for evaluation of cell-based antioxidant protection using erythrocytes, the CAP-e assay. The CAP-e assay was designed to assist the natural products industry when moving from analytical chemistry testing towards more complex biological testing. The method uses assay principles comparable to the Oxygen Radical Absorbance Capacity (ORAC) assay, applies natural products to intact erythrocytes, and specifically measures antioxidants capable Of penetrating into and protecting the cells from free radical damage. The assay can be applied to testing of antioxidant availability to live cells in vitro and documenting antioxidant uptake in vivo. During the past year, we have made progress in several areas, particularly with respect to quality control of the cells used for this assay, including controlling for inter-assay variability.

Poster (pdf)